Protocol for dissection and organotypic explant culture of the neonatal and adult stria vascularis
Protocol for dissection and organotypic explant culture of the neonatal and adult stria vascularis

Protocol for dissection and organotypic explant culture of the neonatal and adult stria vascularis

STAR Protoc. 2025 Oct 30;6(4):104172. doi: 10.1016/j.xpro.2025.104172. Online ahead of print.

ABSTRACT

Comprehensive analysis of the stria vascularis (SV), an essential structure for hearing function, would greatly benefit from an in vitro experimental platform. Here, we present a protocol for dissection and isolation of the neonatal and adult mouse SV (focusing on post-natal day [P]0-35) from base to apex. We then detail procedures for explanting the SV on Matrigel-coated well dishes, followed by washing and fixing procedures. For complete details on the use and execution of the protocol, please refer to Thulasiram et al.1.

PMID:41175367 | DOI:10.1016/j.xpro.2025.104172