STAR Protoc. 2025 Oct 30;6(4):104172. doi: 10.1016/j.xpro.2025.104172. Online ahead of print.
ABSTRACT
Comprehensive analysis of the stria vascularis (SV), an essential structure for hearing function, would greatly benefit from an in vitro experimental platform. Here, we present a protocol for dissection and isolation of the neonatal and adult mouse SV (focusing on post-natal day [P]0-35) from base to apex. We then detail procedures for explanting the SV on Matrigel-coated well dishes, followed by washing and fixing procedures. For complete details on the use and execution of the protocol, please refer to Thulasiram et al.1.
PMID:41175367 | DOI:10.1016/j.xpro.2025.104172