J AOAC Int. 2024 Aug 24:qsae068. doi: 10.1093/jaoacint/qsae068. Online ahead of print.
ABSTRACT
BACKGROUND: Taurine (2-aminoethanesulfonic acid) is an essential β-amino acid which is one of the most abundant intracellular amino acid-like components in humans. The level of free taurine in human milk is higher than in bovine milk. Since taurine is considered important for the development of a newborn, fortification of taurine to infant nutrition is common. This publication describes a rapid method for the determination of taurine in infant formula and adult/pediatric nutritionals.
OBJECTIVE: This publication demonstrates the suitability and applicability of the method to determine taurine content in infant formula and adult/pediatric nutritionals.
METHODS: Test portions are deproteinized by acid in aqueous environment prior to analysis. The level of taurine is analyzed by Hydrophilic Interaction Chromatography (HILIC) coupled with Multiple Reaction Monitoring Mass Spectrometry (MRM-MS). Quantitation is accomplished using a stable isotope labeled internal standard (SILS) for taurine.
RESULTS: The accuracy of the method was validated using standard reference materials and spike recovery studies. The average recoveries ranged between 92-105%. The repeatability and intermediate precision were in the range of 0.7-4.6% (RSDr) and 1.3-6.7% (RSDiR). The limit of quantification was below the requirement of 0.5 mg/100 g.
CONCLUSIONS: The single laboratory validation (SLV) of the method for determination of taurine content in infant formula and adult/pediatric nutritionals demonstrates that it meets the requirements as per AOAC SMPR 2014.013.
HIGHLIGHTS: A fast method to selectively determine the content of taurine in infant formula and adult/pediatric nutritionals was evaluated and found to be fit for purpose for routine product compliance testing. Based on the results of this SLV, the method was approved by the AOAC Stakeholder Program on Infant Formula and Adult Nutritionals (SPIFAN) Expert Review Panel for SPIFAN Nutrient Methods for Official Methods First Action status.
PMID:39180718 | DOI:10.1093/jaoacint/qsae068
