Sleep Adv. 2026 Aug 13;7(3):zpag090. doi: 10.1093/sleepadvances/zpag090. eCollection 2026.
ABSTRACT
STUDY OBJECTIVES: Sleep during adolescence may influence biological aging, yet few studies have examined this association using objective sleep measures. We examined the associations between accelerometry-based sleep characteristics and epigenetic age (EA), epigenetic age acceleration (EAA), and the pace of biological aging.
STUDY METHODS: For this preliminary analysis, we used data from the Health Outcomes and Measures of the Environment Study (Cincinnati, OH). At age 12 years (2016-2019), we used accelerometers to assess total sleep time, sleep efficiency, the number of awakenings, and sleep fragmentation. Using DNA methylation data at the same age, we estimated EA and EAA using the skinbloodHorvath, Hannum, and Wu clocks, and the pace of biological aging using DunedinPACE. We used multivariable linear regression to estimate covariate-adjusted associations between sleep and epigenetic aging. We also evaluated sex modification by including product interaction terms and examined sex-stratified models.
RESULTS: Among 138 adolescents (59 per cent female; mean age 12.29 ± 0.64 years), general trends suggested that poorer sleep quality was associated with higher EA and EAA. However, all effect estimates were imprecise, and confidence intervals (CIs) included the null. Notably, we found that sex modified these relationships: higher sleep fragmentation was associated with elevated EAA among males (Hannum β = 0.885, 95% CI: 0.132, 1.638; interaction p = .005), but not females (β = -0.325, 95% CI: -0.866, 0.217). These associations were consistent across first-generation clocks.
CONCLUSIONS: Overall, we did not find consistent evidence of associations between sleep characteristics and epigenetic aging in this preliminary analysis. However, sex-stratified analyses suggested that poorer sleep quality may be associated with greater EAA among males.
PMID:42732420 | PMC:PMC13570646 | DOI:10.1093/sleepadvances/zpag090
