STAR Protoc. 2025 Aug 28;6(3):104049. doi: 10.1016/j.xpro.2025.104049. Online ahead of print.
ABSTRACT
The success of immunotherapies hinges on identifying targetable cell surface proteins expressed in the cancer of interest. Here, we present a protocol for enriching plasma membrane proteins for mass spectrometry analysis using a density gradient ultracentrifugation approach. We describe steps for cell lysis, membrane isolation, and preparation for downstream analysis. This protocol is applicable to cell lines, cell-/patient-derived xenografts (CDX/PDX), and primary tissues. For complete details on the use and execution of this protocol, please refer to Glisovic-Aplenc et al.,1 Hamilton et al.,2 and Mooney et al.3.
PMID:40884789 | DOI:10.1016/j.xpro.2025.104049
