Mol Biol Rep. 2025 Jun 24;52(1):632. doi: 10.1007/s11033-025-10734-6.
ABSTRACT
PURPOSE: The aim of this study was to evaluate the immunomodulatory effects of methanolic and aqueous extracts of Dalbergia sissoo and Aegle marmelos on immunoglobulin (IgG and IgM) levels in diarrheal calves affected by colibacillosis. The study sought to assess the efficacy of these plant-based extracts in restoring immune competence and enhancing immune function in neonatal calves suffering from infections commonly associated with colibacillosis.
METHODS AND RESULTS: A total of thirty-two colibacillosis-affected calves were randomly assigned into four treatment groups, each consisting of eight calves. Polymerase Chain Reaction results supported the findings of the study. These groups were administered either the methanolic or aqueous extract of A. marmelos(150 mg/kg b.w. twice daily for five days) or D. sissoo leaves (1000 mg/kg b.w. twice daily for five days). In addition, eight healthy calves were selected as controls. The study assessed the immunomodulatory effects by measuring IgG and IgM levels before treatment, on the 3rd day post-treatment, and on the 6th day post-treatment. Statistical analysis revealed significant differences (p < 0.05) between the treatment groups at different time points, demonstrating a marked improvement in immunoglobulin levels after the administration of both A. marmelosand D. sissoo extracts.
CONCLUSION: Results suggest that both methanolic and aqueous extracts of A. marmelosand D. sissoo possess potent immunomodulatory effects that can enhance immune function in diarrheic calves suffering from colibacillosis. The study further underscores the potential therapeutic application of these plant-based extracts in veterinary medicine to restore immune competence in neonatal livestock affected by infections such as colibacillosis.
PMID:40553245 | DOI:10.1007/s11033-025-10734-6
