J Appl Microbiol. 2025 Jun 3:lxaf133. doi: 10.1093/jambio/lxaf133. Online ahead of print.
ABSTRACT
AIMS: Helicobacter pylori (Hp) infection is associated with gastrointestinal and systemic disorders in children. This study compared the effects of triple versus quadruple antibiotic therapies on gut microbiota in children with Hp infection.
METHODS AND RESULTS: Thirty pediatric patients with Hp infection were recruited and randomized into triple or quadruple therapy groups for eradication treatment. Fecal samples were collected before treatment, two weeks, and six weeks post-treatment, followed by 16S rDNA gene sequencing. Baseline gut microbiota showed no significant differences between groups. Triple therapy caused significant disruptions in α-diversity, β-diversity, microbial composition, and metabolic pathways, while quadruple therapy resulted in minimal changes. Post-treatment, quadruple therapy exhibited higher α-diversity, distinct β-diversity, and greater abundance of butyrate-producing bacteria compared to triple therapy. Metabolic pathway analysis also revealed significant differences between the two therapies.
CONCLUSIONS: Triple therapy significantly disrupted gut microbiota balance, whereas quadruple therapy had a milder impact in Hp-infected children, preserving microbial diversity.
PMID:40459911 | DOI:10.1093/jambio/lxaf133
