Mol Biol Cell. 2025 Apr 30:mbcE24110490. doi: 10.1091/mbc.E24-11-0490. Online ahead of print.
ABSTRACT
The PIKfyve-VAC14-FIG4 complex synthesizes and turns over PI(3,5)P2, an essential signaling lipid. A medium-resolution structure revealed that VAC14 forms a star-shaped pentamer scaffold. Two legs of VAC14 bind FIG4, with one leg also occupied by PIKfyve. The significance of VAC14 oligomerization was unknown. Here, using Alphafold2 and cryo-EM maps we generated an atomic-resolution prediction, and found that some mutations linked to pediatric neurodegenerative diseases reside in the VAC14-VAC14 interfaces. A corresponding yeast mutation, along with additional mutations, demonstrate that VAC14 oligomerization is critical for Fab1/PIKfyve function. These mutations cause defects in the generation of PI(3,5)P2, in VAC14 localization, and in VAC14 oligomerization. Similarly, VAC14 patient mutations expressed in human VAC14 knock-out cells, are defective in the formation of the PIKfyve-VAC14-FIG4 complex, as measured by pull-down assays, are defective in VAC14 oligomerization as measured by fluorescence-detection size exclusion chromatography of cell lysates and are defective in co-localization with VPS35-containing endosomes. These studies show that VAC14 oligomerization plays a crucial role in the regulation of PIKfyve/FAB1 and provide insights into selected patient mutations. Moreover, they suggest that small molecules that stabilize the VAC14 complex may provide an intervention for diseases linked to mutations in VAC14.
PMID:40305106 | DOI:10.1091/mbc.E24-11-0490
