STAR Protoc. 2024 Dec 20;6(1):103499. doi: 10.1016/j.xpro.2024.103499. Online ahead of print.
ABSTRACT
The study of somatic mutations in single cells provides insights into aging and carcinogenesis, which is complicated by the dependency on whole-genome amplification (WGA). Here, we describe a detailed workflow starting from single-cell isolation to WGA by primary template-directed amplification (PTA), sequencing, quality control, and downstream analyses. A machine learning approach, the PTA Analysis Toolkit (PTATO), is used to filter the hundreds to thousands of artificial variants induced by WGA from true mutations at high sensitivity and accuracy. For complete details on the use and execution of this protocol, please refer to Middelkamp et al.1.
PMID:39709610 | DOI:10.1016/j.xpro.2024.103499
